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The results obtained for the four known-age (VIRI) bone samples are summarized in Supplementary Table S1.The impact of sample size on the collagen extraction yield and the radiocarbon age are discussed below. In this figure, a normalized yield was calculated for clarity and to enable direct comparisons.
Because they can be identified to the species level and radiocarbon dated, these fossil remains are key to establishing the archaeological chronologies, palaeoenvironmental reconstructions and historical-biogeographical processes (i.e.
post-glacial recolonization events) of the last 50,000 years.
Regarding small vertebrates, only two case studies were found: the Late Prehistoric dispersal of Polynesians to New Zealand was dated using the commensal Pacific rat as a proxy. ivory, bone or antler), progress in sample pretreatments using ultrafiltration.
In both studies, the bones were Late Pleistocene to Holocene in age, and weights were comprised of between 30–60 mg. However, ultrafiltration is often associated with lower extraction yields (especially when bones are moderately to poorly preserved), and does not always allow for the recovery of a sufficient amount of collagen when sample mass is lower than 100 mg. In general, the solution consists in dating a “reliably associated” artefact (often charcoal) from the same stratigraphic unit instead of the bone remains.
Our approach was elaborated on known-age samples from the Fifth International radiocarbon Inter-comparison (VIRI) and served as proof of concept.
The method was then applied to two archaeological sites where the single bones of small mammals were AMS-dated, and the dates compared to standard-size bone samples found in the near vicinity.
An optimized protocol allowed us to extract enough material to produce between 0.2 and 1.0 mg of carbon for graphite targets.
Our approach was tested on known-age samples dating back to 40,000 BP, and served as proof of concept.
The method was then applied to two archaeological sites where reliable dates were obtained from the single bones of small mammals.
These results open the way for the routine dating of small or key bone samples. bones, teeth, antler and ivory) found in the fossil record have a tremendous informative potential relevant to the fields of archaeology, palaeoecology and the history of art and technology.
Collagen was extracted from four macromammal bone samples of known-age (for details please refer to the Methods section) covering the full range of radiocarbon dating: a horse bone VIRI F (less than one C half-life), two whale bones VIRI I and VIRI H (approximately two half-lives) and a mammoth bone VIRI E (more than five half-lives).Tags: Adult Dating, affair dating, sex dating